Techne TC-3000X Manuel d'utilisateur

TC-3000 & TC-3000X OPERATOR'S MANUALIssue 310/08

SICHERHEITS - UND INSTALLATIONSINFORMATIONENSICHERHEIT DES BEDIENPERSONALSLesen Sie diese Anleitung vor Verwendung des Thermocyclers bitte sorgfältig

1. Alle Geräte von Bibby Scientific Ltd werden mit einem Netzkabel geliefert, das entweder eingestecktwird oder fest mit dem Gerät verbunden ist.2. Üb

EINSATZBEDINGUNGENDer Thermocycler ist für den Einsatz unter folgenden Bedingungen ausgelegt:• Gebrauch in Innenräumen• Umgebungstemperatur zwischen

INFORMACIÓN DE SEGURIDAD E INSTALACIÓNSEGURIDAD DEL OPERARIOLea atentamente este manual antes de utilizar el ciclador térmico. Si el equipo no se util

2. Antes de conectar el instrumento al suministro eléctrico, compruebe que el voltaje coincida con elindicado en la placa de régimen (situada en la pa

Nota: Las especificaciones de control corresponden a una temperatura ambiental de 20ºC. Lasespecificaciones pueden empeorar si se utiliza el instrumen

INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONESICUREZZA DELL'OPERATORELeggere attentamente il presente manuale prima di usare il ciclatore t

2. Prima di collegare lo strumento alla rete elettrica controllare il voltaggio riportato sulla etichetta sulretro dell'unità. Assicurarsi che l&

Nota: le specifiche di controllo sono indicate ad una temperatura ambiente di 20°C. Le specifichepotrebbero peggiorare fuori da una temperatura ambien

17BEFORE SWITCHING ONEnsure that the voltage selectorswitch is set to the appropriatevoltage for your supply;On/Off switchEnsure that the appropriate

CONTENTSPAGEINTRODUCTION 1SAFETY AND INSTALLATION 2SÉCURITÉ ET CONSIGNES D'INSTALLATION 5SICHERHEITS- UND INSTALLATIONSINFORMATIONEN 8INFORMAZION

18CONTACT INFORMATIONFor technical, sales or servicing information, contact your local Bibby Scientific Ltd dealer or:UK and Rest of WorldBibby Scient

TC-3000, TC-3000X SPECIFICATIONSTC-3000 TC-3000XTemperatureTemperature range 4°C to 99°C 4°C to 99°CTemperature set point precision 0.1°C 0.1°CBlock u

TC-3000 TC-3000XRunning ProgramsPause button Yes YesStop button Yes YesEnd of program alarm Yes YesAuto restart on power fail Yes YesInterconnectivity

HEATED LID: TC-3000 To release the heated lid, lift the lid catch and thelid will open. To close the lid, push down gentlyuntil the catch clicks in p

TUBES OR REACTION VESSELSBibby Scientific Ltd does not recommend any specific tube or reaction vessel other than those describedin this manual. We rec

0.5ML TUBESThe heated lid mechanism of the TC-3000 with the 0.5ml block is optimised for flat top tubes; it is notsuitable for use with domed cap tube

FRONT PANEL CONTROLSTHE LCD DISPLAYINDICATORSPower: This indicator is lit when there is power to the TC-3000 or TC-3000X.Status: This indicator is lit

The "End" or "Finish" key is used to finish editingprograms or to exit a program. It is also used toexit a sequence of key operati

PROGRAMMING THE TC-3000 & TC-3000XThe table below illustrates the symbols which appear on the LCD display and the relevant keypad buttonto which t

PROGRAMMING OPTIONSThere are two options available for creating programs on the TC-3000 & TC-3000X:1. Copy and edit one of the pre-written templat

INTRODUCTIONThe TC-3000 and larger capacity TC-3000X provide the researcher with the means of accuratelycontrolling the temperature profile of samples

To save the program[ to save ][ YES or NO ]To give a program a nameA new name can now be given to the copiedprogram (see bel

Edit a selected field or stepDONNAHeated Lid 105°CPreheat Lid on[ , to edit ]29To edit a field or step in an existing programWhen the u

[ to disable ][ turn on/off ]30To edit a temperature, type in the requiredtemperature. In the example here, the heatedlid temperature

[ Yes or No ]To delete a selected program31OPTION 2: CREATING A NEW PROGRAMA second option is to create a completely new program without u

EXAMPLE PROGRAMSA couple of example thermal cycling programs are shown below.Typical 3-Step PCR 3-Step PCR with multiple 'Stages'32PROGRAMMI

[ , to edit ][ to delete step ][ , to edit ]To insert additional stepsA new stage will be inserted which consists ofa &a

[ to delete stage ]-------------------------Final Extn 72.0°C 5m00Final hold 10.0°C[ to insert stage ]Use the "Up Arrow" or "Dow

[ ‘.’ to insert step ][ , to edit ]TO PROGRAM INCREMENT OR DECREMENT TEMPERATURE OR TIMEUnder normal circumstances, the Hold Temperature

The Hold Temperature of the incremented/decremented step is the defined Hold Temperature plus thesummation of the increments/decrements. The first cyc

Use the "Alpha/numeric" keys to change thevalues of the 'fst' line to the firsttemperatures and/or times you require.Press "E

SAFETY AND INSTALLATIONOPERATOR SAFETYPlease read this manual carefully before using the thermal cycler. If the equipment is not used in themanner des

At any time during programming the TC-3000 or TC-3000X, when you are satisfied with the program,you can finish and accept what you have done by first

TO RUN A PROGRAM39When the unit is switched on the display willshow this screen.If known, type in the 'Speed dial' number forthe required pr

[ to CONTINUE ][ for info ]During the run the LCD screen will report:1. The cycle number2. The time remaining (counting down insecon

CONFIGURATIONPassword pad[ to accept ]41Program InformationINFORMATIONCONFIGURATIONWarning! The Configuration software should only be ac

42Program defaultsSettingsThere are a number of options which can bechanged; these are listed here.Use the "Up Arrow" and "Down Arrow&q

43The complete list of settings is given here.Auto restart "on" will allow the unit to restartfrom where it left off should there be a power

44Controller resetThe 'Controller reset' will delete all savedprograms from the unit.Highlight 'Controller reset' then press"

45Connecting several TC-3000 units to the computer using the daisy chain cablesIf you are driving two or more units then you will need part number FGE

46Connecting several TC-3000X units to the PC.If you are driving two or more units then you will need a USB cable for each unit, part number 6205046pl

47NOTES ON TRANSFERRING PROTOCOLS FROM OTHER THERMAL CYCLERSThe TC-3000 and TC-3000X can heat up to 3.6°C/s and provides the highest levels of tempera

the On/Off switch) is set to the appropriate voltage for the local supply. Please note that the unit mustbe earthed to ensure proper electrical safety

48USER MAINTENANCECLEANING YOUR TC-3000 OR TC-3000XBefore cleaning your unit, disconnect from the power supply and allow it to cool to below 50°C. CLE

49SOME FREQUENTLY ASKED QUESTIONSQ1 What is the 'Pause' function at the start of the program used for? A1 Some users prefer to preheat the h

50ADDITIONAL INFORMATIONBrief fault finding notes and a list of replacement parts are given in this section.Note that this equipment should only be di

Underside of the TC-3000(TC-3000X is identical )ACCESSORIESThe following accessories can be obtained from Bibby Scientific Ltd or your Bibby Scientifi

REPLACEMENT PARTS The following replacement parts can be obtained from Bibby Scientific Ltd or your Bibby Scientific Ltddealer:Item No Description Qua

Bibby Scientific LtdBeacon Road Stone Staffordshire ST15 0SAUnited KingdomTel: +44 (0)1785 812121 Fax: +44 (0)1785 813748 email: techne@bibby-sc

4GUARANTEEThe instrument is guaranteed against any defect in material or workmanship for the period specified onthe enclosed guarantee card. This peri

SÉCURITÉ ET CONSIGNES D'INSTALLATIONSÉCURITÉ DE L’OPÉRATEURLire attentivement le présent manuel avant d'utiliser le cycleur thermique. Si ce

1. Tous les appareils Bibby Scientific Ltd sont livrés avec un câble d'alimentation, qui peut être intégré àl'appareil ou muni d'une fi

Remarque: Les caractéristiques de régulation sont indiquées à une température ambiante de 20°C. Cescaractéristiques peuvent se détériorer en dehors d&