Techne TC-3000X Manuel d'utilisateur télécharger pdf (Page 29)

PROGRAMMING OPTIONS

There are two options available for creating programs on the TC-3000 & TC-3000X:

1. Copy and edit one of the pre-written templates.

2. Create a new program from the defaults.

OPTION 1: CREATING PROGRAMS USING TEMPLATES

There are two pre-written basic PCR templates: a two-step and a three-step template. These can be

copied and modified as required.

Other templates available include Ice bucket (incubation at 4ºC), Ligation (incubation at 15ºC) and an RT-

PCR template.

Use the "Down Arrow" key to select an

existing 'Template' program from the menu,

e.g. '2 Step Template' or '3 Step Template'.

Press the "Enter" key to accept it.

The Template is already defined and can be run

as it is or easily copied, modified and saved

under a new name.

Use the "Down Arrow" key to select 'Copy

Program 'Press the "Enter" key to accept it.

2 STEP TEMPLATE [501]

Run program

View program

[ , to select ]

2 STEP TEMPLATE

Heated Lid 105°C

Preheat Lid on

Pause off

-------------------------

In denat 94°C 5m00

Hot Start off

------- Stage 1---------

Number cycles 30

Step 94.0°C 0m30 max

Step 68.0°C 1m00 max

-------------------------

Final Extn 72.0°C 5m00

Final hold 10.0°C

3 STEP TEMPLATE

Heated Lid 105°C

Preheat Lid on

Pause off

-------------------------

In denat 94.°C 5m00

Hot Start off

------- Stage 1---------

Number cycles 30

Step 94.0°C 0m30 max

Step 55.0°C 0m30 max

Step 72.0°C 0m30 max

-------------------------

Final Extn 72.0°C 5m00

Final hold 10.0°C

Run program

View program

Copy program

[ , to select ]

* * * New Program * * *

2 STEP TEMPLATE 501

3 STEP TEMPLATE 502

[ , to select ]

1 2 ... 24 25 26 27 28 29 30 31 32 33 34 ... 54 55

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Techne TC-3000X Manuel d'utilisateur Page 29

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