Techne TC-3000G Manuel d'utilisateur

TC-3000G OPERATOR'S MANUALIssue 26/09

SICHERHEITS - UND INSTALLATIONSINFORMATIONENSICHERHEIT DES BEDIENPERSONALSLesen Sie diese Anleitung vor Verwendung des Thermocyclers bitte sorgfältig

1. Alle Geräte von Bibby Scientific Ltd werden mit einem Netzkabel geliefert, das entweder eingestecktwird oder fest mit dem Gerät verbunden ist.2. De

GARANTIEBibby Scientific Ltd gewährleistet, dass dieses Gerät für den auf der Garantiekarte angegebenen Zeitraumkeine Herstellungs- und Materialmängel

INFORMACIÓN DE SEGURIDAD E INSTALACIÓNSEGURIDAD DEL OPERARIOLea atentamente este manual antes de utilizar el ciclador térmico. Si el equipo no se util

2. EI TC-3000G tiene una fuente conmutada que es conveniente para el uso con voltajes de entradauniversales de CA entre 90 y 260V. Conecte el cable de

GARANTÍAEl instrumento está garantizado contra cualquier defecto en el material o la fabricación durante elperíodo especificado en la tarjeta de garan

INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONESICUREZZA DELL'OPERATORELeggere attentamente il presente manuale prima di usare il ciclatore t

2. Il modello TC-3000G dispone di alimentazione idonea per voltaggi in corrente alternata tra 90 e260V. Collegare il cavo di alimentazione ad una spi

GARANZIA Lo strumento è garantito da qualsiasi difetto nei materiali o nella lavorazione per il periodo specificatonella scheda di garanzia allegata.

17CONTACT INFORMATIONFor technical, sales or servicing information, contact your local Bibby Scientific Ltd dealer or:UK and Rest of WorldBibby Scient

CONTENTSPAGEINTRODUCTION 1SAFETY AND INSTALLATION 2SÉCURITÉ ET CONSIGNES D'INSTALLATION 5SICHERHEITS- UND INSTALLATIONSINFORMATIONEN 8INFORMAZION

TC-3000G SPECIFICATIONTemperatureGradient range 20°C to 80°CMaximum gradient 15°CMinimum gradient 1°CTemperature range 4°C to 99°CTemperature set poin

ProgrammingProgram naming using alpha keys YesProgram password protection YesNumber of programs (3 step) 80Maximum number of steps per stage 35Maximum

HEATED LID The heated lid has a rotating knob to adjust the lidpressure on the samples, allowing for a variety ofconsumables to be used. To adjust the

0.5ML TUBESIn order to fit the maximum number of tubes in the block, they need to be placed so that all the hingesare in the same relative orientation

FRONT PANEL CONTROLSTHE LCD DISPLAYINDICATORSPower: This indicator is lit when there is power to the TC-3000G.Status: This indicator is lit when the u

The "End" or "Finish" key is used to finish editingprograms or to exit a program. It is also used toexit a sequence of key operati

PROGRAMMING THE TC-3000GThe table below illustrates the symbols which appear on the LCD display and the relevant keypad buttonto which they refer.In t

PROGRAMMING OPTIONSThere are two options available for creating programs on the TC-3000G:1. Copy and edit one of the pre-written templates.2. Create a

To save the program[ to save ][ YES or NO ]To give a program a nameA new name can now be given to the copiedprogram (see bel

Edit a selected field or stepDONNAHeated Lid 105°CPreheat Lid on[ , to edit ]27To edit a field or step in an existing programWhen the u

INTRODUCTIONThe TC-3000G provides the researcher with the means of accurately controlling the temperature profileof samples. It has many scientific ap

[ to disable ][ turn on/off ]28To edit a temperature, type in the requiredtemperature. In the example here, the heated lidtemperature

[ Yes or No ]To delete a selected program29OPTION 2: CREATING A NEW PROGRAMA second option is to create a completely new program without u

EXAMPLE PROGRAMSA couple of example thermal cycling programs are shown below.Typical 3-Step PCR 3-Step PCR with multiple 'Stages'30PROGRAMMI

[ , to edit ][ to delete step ][ , to edit ]To insert additional stepsA new stage will be inserted which consists ofa &a

[ to delete stage ]-------------------------Final Extn 72.0°C 5m00Final hold 10.0°C[ to insert stage ]Use the "Up Arrow" or "Dow

[ ‘.’ to insert step ][ , to edit ]33To insert a pause after an existing stage,follow the steps for inserting a stage asabove.Highlight

34123456ABCDEFGHSet temperatureHighesttemperatureLowesttemperatureGradientTO PROGRAM A GRADIENTA gradient of temperatures across the block can be usef

35A gradient calculator is included in the software which can be used to display more accurately thetemperatures of the block for any given gradient.

36Step 94.0°C 00m30 max Step 75.0° C 00m30 maxgradient 10.0°C[ gradient reduced ]Step 94.0°C 00m30 max Step 75.0° C 00m30 maxgradie

The Hold Temperature of the incremented/decremented step is the defined Hold Temperature plus thesummation of the increments/decrements. The first cyc

SAFETY AND INSTALLATIONOPERATOR SAFETYPlease read this manual carefully before using the thermal cycler. If the equipment is not used in themanner des

38Use the "Up Arrow" or "Down" Arrow"keys to move to the step where you want anincrement or decrement temperature or timePres

39Step 94.0° C 00m30 maxfst 65.0° C 00m30 maxlst 55.0° C 1m30 [ edit time ]When you run the program it will automatically increm

TO RUN A PROGRAM40When the unit is switched on the display willshow this screen.If known, type in the 'Speed dial' number forthe required pr

[ to CONTINUE ][ for info ]During the run the LCD screen will report:1. The cycle number2. The time remaining (counting down insecon

CONFIGURATIONPassword pad[ to accept ]42Program InformationINFORMATIONCONFIGURATIONWarning! The Configuration software should only be ac

43Program defaultsSettingsThere are a number of options which can bechanged; these are listed here.Use the "Up Arrow" and "Down Arrow&q

44The complete list of settings is given here.Auto restart "on" will allow the unit to restartfrom where it left off should there be a power

45Controller resetThe 'Controller reset' will delete all savedprograms from the unit.Highlight 'Controller reset' then press"

GRADIENT CALCULATORIt is not possible to view the actual temperatures of each column in the progam or while the unit isrunning. Therefore for any set

47Connecting several TC-3000G units to a PC.If you are driving two or more units then you will need a USB cable for each unit, part number 6205046plus

2. Before connecting the instrument to the mains electricity supply, check the voltage against the ratingplate (located on the back of the unit). Plea

48NOTES ON TRANSFERRING PROTOCOLS FROM OTHER THERMAL CYCLERSThe TC-3000G can heat up to 3.3°C/s and provides the highest levels of temperature uniform

49USER MAINTENANCECLEANING YOUR TC-3000GBefore cleaning your unit, disconnect from the power supply and allow it to cool to below 50°C. CLEANING YOUR

50SOME FREQUENTLY ASKED QUESTIONSQ1 What is the 'Pause' function at the start of the program used for? A1 Some users prefer to preheat the h

51ADDITIONAL INFORMATIONBrief fault finding notes and a list of replacement parts are given in this section.Note that this equipment should only be di

Underside of the TC-3000GACCESSORIESThe following accessories can be obtained from Bibby Scientific Ltd or your Bibby Scientific Ltd dealer:Item No De

REPLACEMENT PARTS The following replacement parts can be obtained from Bibby Scientific Ltd or your Bibby Scientific Ltddealer:Item No Description Qua

Bibby Scientific LtdBeacon Road Stone Staffordshire ST15 0SAUnited KingdomTel: +44 (0)1785 812121 Fax: +44 (0)1785 813748 email: techne@bibby-sc

4GUARANTEEThe instrument is guaranteed against any defect in material or workmanship for the period specified onthe enclosed guarantee card. This peri

SÉCURITÉ ET CONSIGNES D'INSTALLATIONSÉCURITÉ DE L’OPÉRATEURLire attentivement le présent manuel avant d'utiliser le cycleur thermique. Si ce

1. Tous les appareils Bibby Scientific Ltd sont livrés avec un câble d'alimentation, qui peut être intégré àl'appareil ou muni d'une fi

GARANTIEL'appareil est garanti contre tout défaut de matériaux ou vice de fabrication pendant la période préciséesur la carte de garantie jointe.