Techne TC-3000G Manuel d'utilisateur télécharger pdf (Page 22)

HEATED LID

The heated lid has a rotating knob to adjust the lid

pressure on the samples, allowing for a variety of

consumables to be used. To adjust the pressure:

• Adjust the orange rotating knob anticlockwise

to give the least pressure.

• Place the samples in the block and place the lid

in the down position.

• Gently rotate the knob clockwise until you can

feel pressure beginning to be applied.

• Rotate the knob a further quarter of a turn and

this will give the correct pressure.

USER OPERATION AND CONSUMABLES

Do not over-tighten the knob as this will cause deformation of the silicone rubber mat (where used) and

reduce the effectiveness of the lid. Once the pressure has been set it does not need to be adjusted unless

a different block or consumable is used.

The silicone rubber mat supplied with the unit (Part number 6106196) is for use with 48 well microplates

when sealing using a plastic adhesive film. The mat should be placed on top of the sealing film to ensure

good thermal contact with the heated lid.

TUBES OR REACTION VESSELS

Bibby Scientific Ltd does not recommend any specific tube or reaction vessel other than those described

in this manual. We recommend using reaction volumes between 20 and 200µl. The tubes must withstand

a pressure of 1 atmosphere at 100°C. Any vessel must be able to withstand the temperatures you are

using without any danger of them deforming to the point where they fracture.

To test your tubes, put 25µl of water into each of 5 tubes and subject them to a typical thermal cycling

protocol. At the end of the program, measure the volume remaining using a micropipette. A loss of more

than 1 or 2µl indicates a vapour leak.

The amount of volume loss you observe and the change in reactant concentrations you can tolerate

determine the minimum volume that can be used. Typical volume losses of 1µl in 30 cycles allow the use

of samples of 20µl or less.

During the final cool-down, a ring of condensation may form above the liquid level but below the top of

the sample block. This is normal and does not effect the reaction.

1 2 ... 17 18 19 20 21 22 23 24 25 26 27 ... 55 56

Pas de commentaire

Techne TC-3000G Manuel d'utilisateur Page 22