Techne TC-3000G Manuel d'utilisateur télécharger pdf (Page 32)

EXAMPLE PROGRAMS

A couple of example thermal cycling programs are shown below.

Typical 3-Step PCR 3-Step PCR with multiple 'Stages'

PROGRAMMING

To add a stage and then edit it

Heated Lid 105°C

Preheat Lid on

Sample cooling on

Pause off

-------------------------

In denat 94.0°C 5m00

Hot Start off

-------- Stage 1 --------

Number cycles 30

Step 94.0°C 0m30 max

Step 55.0°C 0m30 max

Step 72.0°C 0m30 max

-------------------------

Final Extn 72.0°C 5m00

Final hold 10.0°C

Heated Lid 105°C

Preheat Lid on

Sample cooling on

Pause off

-------------------------

In denat 94.0°C 5m00

Hot Start off

-------- Stage 1 --------

Number cycles 10

Step 94.0°C 0m30 max

Step 60.0°C 0m30 max

Step 72.0°C 0m30 max

-------- Stage 2 --------

Number cycles 20

Step 94.0°C 0m30 max

Step 55.0°C 0m30 max

Step 72.0°C 0m30 max

-------------------------

Final Extn 72.0°C 5m00

Final hold 10.0°C

Create a new program by selecting ** New

Program ** from the menu and press

"Enter"

Give the program a name and edit any of the

default fields as described above.

Use the "Down Arrow" key to scroll to

the dotted line below 'Hot Start'; it will start

to flash.

Press "Enter" to insert a stage.

* * New Program * * [ ]

2 STEP TEMPLATE 501

3 STEP TEMPLATE 502

[ , to select ]

UNNAMED #

Heated Lid 105°C

Preheat Lid on

[ , to edit ]

In denat 94.0°C 5m00

Hot Start off

-------------------------

[ to insert stage ]

[ to save ]

1 2 ... 27 28 29 30 31 32 33 34 35 36 37 ... 55 56

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Techne TC-3000G Manuel d'utilisateur Page 32

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